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Original paperImmunohistochemical Analysis
of Cyclin D1 Expression in Low Grade B-Cell non Hodgkin Lymphomas
Darko Vugdelija
Internistička služba, Zdravstveni centar Zaječar
Summary:
Non Hodgkin lymphomas (NHL) represent diversity group of clonal
diseases, which arises from lymphocyte precursors in different stage of
development. Classification of non Hodgkin lymphomas has dramatically
evolved in past century. Immunohistochemical analyses with monoclonal
antibodies led to major breakthrough in subdivision and diagnoses of
NHL. Cyclin D1 represent product of bcl-1 gen, which over expression is
often a conequvence of reciprocal translocation t(11;14)(q13;q32). It
has major role in cell cycle control, driving cell into S phase by
complexing with cyclin dependent kinases (cdk). Cyclin D1 immunostaining
can differentiate morfologicaly similar lymphomas with divergent
clinical behaviour. Aim of our work was to analyse cyclin D1 expression
by immunohistohemical staining in patients with low grade NHL. We have
analysed cyclin D1 expression in 62 patients: 21 with mantle cell
lymphoma (MCL), 20 with folicular lymphoma (FL) and 21 with B-cell
chronic lymphocitic leukaemia (CLL) using LSAB and CSA staining methods.
We used Hi-square test for statistical analyses. RESULTS: 85,7% of MCL
were cyclin D1 positive, 10% of FL and 4,8% of CLL. Cyclin D1 was over
expressed in MCL with statistically highly significant difference toward
FL (χ2=23,504; p=0,000) and CLL (χ2=27,776; p=0,000). CONCLUSION: These
findings demonstrate that immunostaining for cyclin D1 protein
expression is useful in distinguishing MCL from other B-cell lymphoid
neoplasms.
Key words: cyclin-D1, mantle cell lymphoma, immunohystochemistry |
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